HPLC-Autosampler S5200

With the next upgrading step, the S5200 can be used for fully automatic sample processing for precolumn derivatisation. In the derivatisating mode, up to three independent reagents can be added to a specified sample volume, mixed with the sample and programmed with independent reaction times.

In addition, the sample can be diluted with more than 100 times (depending on sample volume and syringe volume). For temperature sensitive samples, the sample tray can be cooled or heated with the optional cooling system.

For chemical inert operation the instrument can be delivered for fully metal-free sample handling (completely equipped with PEEK and FEP materials, including the sample needle).
A wide range of sample racks and sample vials can be used for operation from 1ml up to 5000 ml or by using micro filter plates. Simple operation through large alphanumeric display menu.

Injection modes	1) Fixed volume (standard) with 20 μl sample loop 2) Variable volume (1st grade option) 100 μl sample loop 3) Sample processing (2nd grade option) 100 μl sample loop
Metering device	Standard: 500ml syringe stepper motor driven with a resolution of 35 steps/ 1 μl. Optional: Syringe resolution                  100 ml      175 steps / 1 μl                  250 ml       70 steps / 1 μl                1000 ml       15 steps / 1 μl                2500 ml        6 steps / 1 μl                5000 ml        3 steps / 1 μl
Injection volume	Fix         : 1,0 to 9,9 times sample loop volume in 0,1 increments programmable Variable : 1µl - 5000µl in 1µl increments
Sample processing	3 reagents each one 1 μl to 5000 μl in dependently programmable 3 reaction times independently programmable needle wash after each processing step programmable sample mixing after each processing step 1-9 times programmable
Sample diluting	up to 5000 μl diluting solvent in 1 μl increments can be added to the sample volume mixing after diluting 1 - 9 time programmable
Needle wash	Through purge position or inject position selectable Wash program for selecting needle wash activity and washing volume 1 - 9 times of the syringe volume
Reproducibility	less than 1% upon injection of 10 μl variable volume less than 0.5% upon 100 μl variable volume and fix volume injection
Memory effect Injection / Vial Analysis time	less than 0.01% depending on selected wash program 1 to 9 different volumes programmable for each injection up to 9999 programmable time base selectable in 1 minute, 0.1 min or in seconds
Sample trays	Standard: 2 pcs. for 60 vials /each total 120 for 1.5ml glass vials or for plastic conical Eppendorf types Optional: preparative version: 2pcs. for 24 vials /each for 5ml glass vials micro filter plates holder
Option	cooling - heating system minimum: Rt - 20°C up to 70°C PEEK - Version (chemically inert option including the needle)

Cat.-No.	Description
S5200	Standard Autosampler S5200, fix volume (20µl) injection, max. 120 vials
Cat.-No.	Description
S5200 - 01 S5200 - 02 S5200 - 03 S5200 - 04 S5200 - 013	Variable Injection Volume (1st grade option), 100 μl sample loop, max. 120 vials Variable Sample Processing (2nd grade option) 60 sample/60 sample processing vials Cooling (RT- 20°C) / Heating System (max 70°C) upgrade for S5200 Preparative injection upgrade f. S5200, fix volumn 1ml (Standard), max. 48 vials a 5ml PEEK - Version (chemically inert option, including PEEK - needle)