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ZAM 3000 Applications

Higher Alcohols
Compounds Tetradecanol
Hexadecanol
Octadecanol
Column Inertsil ODS 3, 40 x 4 mm
Solvent Water / Acetonitrile 5 / 95
Flow: 1 mL/min
ELSD Detection Temperature: 50 °C
Gas pressure (nebulizer): 1.0 bar
Gas flow additive gas: 1.5 L/min
Higher Alcohols
Phospholipids
Compounds Phosphatidyl choline
Lysophosphatidyl choline
Column Merck Lichrospher 100diol-5µ
125 x 4 mm
Column Temperature: 40 °C
Solvent Gradient elution
A: n-Hexane / 2-Propanol / Acetic acid / Triethylamine
B: Water / 2-Propanol / Acetic acid / Trietylamine
ELSD Detection Temperature: 50 °C
Gas pressure (nebulizer): 1.0 bar
Gas flow additive gas: 1.7 L/min
Phospholipids
Organic Acids
Compounds Malic acid / Succinic acid / Fumaric acid
Column InertSil ODS-3, 5µm, 250 x 4.6 mm
Column Temperature: ambient
Solvent 0.01% Trifluoro acetic acid (TFA) in 90/10 Methanol/Water
ELSD Detection Temperature: 50 °C
Gas pressure (nebulizer): 1.6 bar
Gas flow additive gas: 1.5 L/min
Organic Acids
Sugars
Compounds Phosphatidyl choline
Lysophosphatidyl choline
Column Merck Lichrospher 100diol-5µ
125 x 4 mm
Column Temperature: 40 °C
Solvent Gradient elution
A: n-Hexane / 2-Propanol / Acetic acid / Triethylamine
B: Water / 2-Propanol / Acetic acid / Trietylamine
ELSD Detection Temperature: 50 °C
Gas pressure (nebulizer): 1.0 bar
Gas flow additive gas: 1.7 L/min
Sugars
Polystyrene Standard Mixture
Compounds Polystyrene Standards
18700, 8300, 2360
Column Jordi Gel DVB 500A, 500 x 10 mm
Column Temperature: ambient
Solvent THF
Flow: 1.0 mL/min
ELSD Detection Temperature: 35 °C
Gas pressure (nebulizer): 1.0 bar
Gas flow additive gas: 1.1 L/min
Polystyrene Standard Mixture
Urea
Compounds Urea 5 mg/100 mL
in 80/20 Water/Acetonitrile
Column %
Solvent 80/20 Acetonitrile/Water
ELSD Detection Temperature: 25 °C
Gas pressure (nebulizer): 1.0 bar
Gas flow additive gas: 1.1 L/min
Urea
Polyethylene glycol
Compounds Polyethylen Glycol in Methanol
Column OptoSil ODS-2, 5µm, 300 x 4 mm
Column Temperature: ambient
Solvent Gradient Elution:
A: Water / Methanol 80/20
B: Water / Methanol 50/50
ELSD Detection Temperature: 50 °C
Gas pressure (nebulizer): 1.0 bar
Gas flow additive gas: 1.5 L/min
Polyethylene glycol